Practical Bioinformatics is an accessible guide for graduate students studying bioinformatics, biotechnology, and other related subdisciplines. The authors aim to not only provide a practical guide to bioinformatics, but also to help them optimize parameters for data analysis and draw accurate concludions from the reusults. In addition to parameter optimization, the text will also familiarize students with relevant terminology.
The text will approach the basics of bioinformatics, particularly downloading molecular sequences (nucleotide and protein) from databases, BLAST analyses, primer designing and its quality checking, multiple sequence alignment (global, local and freely available tools), phylogenetic tree construction using different algorithms (UPGMA, NJ, MP, ME, FM algorithm) using MEGA6 suite, prediction of protein structures and genome annotation.